Double-staining artefact observed in certain individuals during dual-colour immunophenotyping of lymphocytes by flow cytometry
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چکیده
منابع مشابه
Standardizing Flow Cytometry Immunophenotyping Analysis from the Human ImmunoPhenotyping Consortium
Standardization of immunophenotyping requires careful attention to reagents, sample handling, instrument setup, and data analysis, and is essential for successful cross-study and cross-center comparison of data. Experts developed five standardized, eight-color panels for identification of major immune cell subsets in peripheral blood. These were produced as pre-configured, lyophilized, reagents...
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OBJECTIVE To provide an overview of the utility of flow cytometry for phenotyping of acute leukaemias and selection-of monoclonal antibodies. DATA SOURCES The literature review was obtained through internet, journals and chapters in the relevant books. DATA SELECTION Relevant articles and chapters on immunophenotyping of acute leukaemias were selected from respected international journals a...
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T he conventional laboratory diagnosis of lymphoma using tissue biopsies is based on histology diagnosis and immunohistochemistry. However, this method is tedious and involves multiple steps and procedures. In developed countries, immunophenotyping by flow cytometry is readily performed on tissue biopsies such as lymph nodes for diagnostics purposes. However, the scenario is different in Malays...
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A total of 97 acute promyelocytic leukemia (APL) patients with adequate flow cytometry (FC) data, bone marrow aspirates and presence of t(15;17)/PML-RARA by cytogenetics and/or FISH studies were analyzed for immunophenotypic pattern. Leukemic cells had the following phenotype: CD11b-, CD11c-, CD13+, CD33+, CD45+, CD64+/-, CD117+, and HLA-DR-. A subset of cases showed also an expression of CD2, ...
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Background: Chromomycin A3 (CMA3) staining, with the slide method or fluorescence microscopy, is widely used for indirect assessment of protamine deficiency in a semen sample. Flow cytometry is the most suitable tool which to improve assessment accuracy, both in terms of statistical analysis and to prevent observer variation. This study provides a simple procedure to account for merocyanine 540...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1993
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.990140613